Methods and experimental techniques

In our work, we use the following methods and experimental techniques:

  • FLIM (Fluorescence-Lifetime Imaging Microscopy) used as an imaging technique in confocal microscopy.
  • Single-photon timing technique for lifetime measurements of the excited-state molecules.
  • Transient absorption and diffuse-reflectance laser flash photolysis for spectral and kinetic measurements of short-living transient species, such as excited-state molecules, free radicals, radical-ions, and reactive complexes.
  • Time-resolved singlet oxygen luminescence experiments, to evaluate the photosensitizing efficiency of various compounds.
  • Laser-induced fluorescence emission measurements, to study time-resolved excited-state dynamics in crystal solids.
  • Ground-state diffuse reflectance absorption spectra to study the radiation absorbance properties in opaque and strongly scattering systems.
  • Total luminescence spectroscopy to evaluate the efficiency of the excited state formation.
  • Elemental analysis to study the molecular composition.
  • 1H NMR and 13C NMR, to study the molecular structure.
  • Infrared and near-infrared spectroscopy, to study the molecular structure and monitor chemical changes.
  • X-ray diffraction analysis, to study crystal structure.
  • Electrospray ionization mass spectrometry (ESI-MS) and electron-impact mass spectrometry (EI-MS), to study the mass-spectra and molecular structure of large molecules.
  • Quantum-chemical calculations using different levels of the DFT method are performed to provide a theoretical understanding of various molecular properties, and to predict such properties in novel molecules.



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